1 x Bottle HEPES Buffered Solution, 500 mL. Keep up to speed on the latest scientific developments, events, tips and tools from Lonza. to you upon submission of this form. Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. By continuing to browse the site, you are agreeing to our use of cookies. Questions? HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. A zwitterionic N-substituted aminosulfonic acid buffer. Reproduction of any materials from the site is strictly forbidden without permission. IBMX, Empirical Formula (Hill Notation): C10H14N4O2, Synonym: If you find a lot number with a filling-code such as HEPES Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. HEPES Buffer Solution is used as a common buffer in cell culture media. HEPES has been described as one of the best all-purpose buffers available for biological research. number 09019TO without the first two letters. 6′-(Diethylamino)-4′-(fluoromethyl)spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3′-yl β-D-galactopyranoside, Empirical Formula (Hill Notation): C31H34FNO8, Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid). We offer a 100% guarantee on all our products. Lower concentrations are usually not adequate to control fluctuations in pH, and higher concentrations are often toxic. All Rights Reserved. Sorry we cannot compare more than 4 products at a time. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. There are currently no reviews for this product. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application. HEPES solution, xNa+, Molecular Weight: 970.99 (free acid basis), Synonym: Sigma-Aldrich pricing When Should I Use HEPES in Cell Culture Media? Add some to cart or login. Sorry we cannot compare more than 4 products at a time. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt. HEPES has been used in a wide variety of applications, including tissue culture. HEPES BSS at a fill volume of 100 mL is a component of the ReagentPackTM Subculture kit (catalog number CC-5024). | Privacy. | Privacy. Has a pKa of 7.48 at 25°C. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt, Anti-phospho-AMPA Glutamate Receptor 1 (pSer. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid-d, PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration), BioUltra, for molecular biology, ≥99.5% (T), Pharmaceutical Secondary Standard; Certified Reference Material, BioPerformance Certified, suitable for cell culture, ≥99.5%, ≥99.5% (titration), free-flowing, Redi-Dri, 1M, BioReagent, suitable for cell culture, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. 1-(1,2-Dihexadecanoylphosphatidyl)inositol-3,4-diphosphate sodium salt, Phosphatidylinositol-3,4-diphosphate C-16 sodium salt, 3,7-Dihydro-1-methyl-3-(2-methylpropyl)-1, 6′-(Diethylamino)-4′-(fluoromethyl)spiro[isobenzofuran-1(3. The DMEM/F12, with HEPES, L-Glutamine is available in a 500 mL format & has been optimized & validated for Stem cell culture. The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others. Need a break from your daily routine? following the words 'Lot' or 'Batch'. NACRES NA.25 HEPES Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. Contact Customer Service, Synonym: Has a pKa of 7.48 at 25°C. A buffer solution of HEPES can be prepared by any of several methods. It is also a component of culture medium RPMI• As a component of dissection buffer for culturing rat cortical neurons• Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses• As a component of Danieu′s aquarium water used in agarose gel preparation. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. -021. It is commonly used to buffer cell culture media in air. Lot and Batch Numbers can be found on a product's label WHERE SCIENCE INTERSECTS INNOVATIONTM. A zwitterionice N-substituted aminosulfonic acid buffer. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. Pricing & availability is not currently available. review HEPES Buffer Solution (1 M) and earn rewards! STBB0728K9 - enter the lot number STBB0728 without the filling-code K9. Bio-Techne HEPES buffer (1 M HEPES-NaOH, pH 7.5) Recipe | Mar 21, 2013 Recommendations: n/a. | Privacy. Since the buffering capacity of HEPES is independent of the CO2 concentration, it is an ideal buffer for maintaining the pH of cultures when cell cultures require extended periods of manipulation outside of a CO2 incubator.

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