0000003222 00000 n 0000043456 00000 n 0000002456 00000 n 0000126809 00000 n 112 0 obj <> endobj Find out more about this exciting new development, Using our new visualization tools you can, Using our new highlighting and annotation tool you can, remove selected records that are not saved in My CABI, sign you out of your [1][2] Based on these descriptions of the cell, Gluconacetobacter diazotrophicus can be classified with the bacillus genus. If you would like to, you can learn more about the cookies we use. [2] The bacterium has been studied to move from different crops and also helpful in growing corn. 0000053486 00000 n 0000128205 00000 n 0000042681 00000 n Overall, Gluconacetobacter diazotrophicus, through the research restated, plays a significant role in the environment for plants specifically sugar cane, helps to grow crops, and can be found in areas that are acidic and contain oxygen. 15.5) and could grow on high sucrose concentrations (10%) and fix N 2. [1] Because they create phytohormones, Gluconacetobacter diazotrophicus has proven to stimulate other plant’s growth. [2] Gluconacetobacter diazotrophicus is a notable microbe because studies have shown that the bacteria can help tomatoes and other crops grow. 0000058998 00000 n 0000004192 00000 n 0000127076 00000 n Gluconacetobacter- like diazotrophs were encountered as dense populations inside the root and stem tissues of sugarcane cultivated in ancient agricultural fields of the Nile Delta. [9] In regard to the ecology of this microorganisms, the numbers of G. diazotrophicus that colonize sugarcane decrease when the plant is grown under high nitrogen fertilization doses. [2] The G-C content for Gluconacetobacter diazotrophicus could be calculated at 66.19%. 0000033700 00000 n 0000015593 00000 n There are over 13,605,000 records available in CAB Direct | Last updated on November 26, 2020. 0000065511 00000 n [2][3], Originally found in Alagoas, Brazil, Gluconacetobacter diazotrophicus is a bacterium that has several interesting features and aspects which are important to note. It was widely studied and used as a model system to assess the bacterial endophyte – plant interactions. 0000037350 00000 n 0000005279 00000 n a convenient, single point of access to all of your CABI database subscriptions. Gluconacetobacter diazotrophicus is a very well-known and well-studied member of family Acetobacteraceae. The bacterium is known for stimulating plant growth and being tolerant to acetic acid. [2] This genome has one circular chromosome and two plasmids. 0 0000127391 00000 n Counts of >10 5 cells g -1 were recorded in root and stem samples. 114 0 obj<>stream [2] The bacterium was first discovered by Vladimir A. Cavalcante and Johanna Dobereiner while analyzing sugarcane in Brazil. 0000007184 00000 n [2][3] Again, the bacterium’s name was changed to Gluconacetobacter diazotrophicus when its taxonomic position was resolved using 16s ribosomal RNA analysis. 0000004969 00000 n [5][6] After overnight growth at 301C, QIAGEN blood and cell culture DNA Midi kit … 0000042037 00000 n [3] The cells be viewed as darkish brown or orange colonies under a microscope. 0000126629 00000 n 0000005902 00000 n 0000036455 00000 n 0000039542 00000 n For DNA isolation, a single bacterial colony of isolate DS1 was inoculated into 5ml of a rich medium (gl 1; glucose, 2.0; glutamic acid, 1.5; peptone, 1.5; K 2HPO 4, 0.5; MgSO 4 7H 2O, 0.5; yeast extract, 2.0; pH, 6). Gluconacetobacter diazotrophicus is a bacterium with a rod-like shape, has circular ends, and can be classified as a Gram-negative bacterium. 0000034063 00000 n [2] Because the bacterium is found on sugar cane when first discovered, as explained prior, and reacts strongly to high amounts of sugar, Gram Negative is the correct classification for Gluconacetobacter diazotrophicus. 0000005046 00000 n 0000036832 00000 n 0000005203 00000 n 0000003829 00000 n 0000004418 00000 n 0000016660 00000 n 0000040252 00000 n x�b```f`��e`g`��� Ȁ �l@Q�U0��o�t�Gr��� Gluconacetobacter diazotrophicus cells are shaped like rods, have ends that are circular or round, and have anywhere from one to three flagella that are lateral. 0000127496 00000 n 0000051022 00000 n 0000038428 00000 n [7] Besides to be a nitrogen-fixing bacterium, G. diazotrophicus synthesizes Indole-3-acid acetic, that could contribute to promote the growth of the associated plant. 0000058762 00000 n 0000126496 00000 n 0000005124 00000 n This is to ensure that we give you the best experience possible. %PDF-1.4 %���� Strains of this species have been isolated from all over the world. 0000127635 00000 n Bacterial cultures have been preserved in 20% glycerol at −80 °C prior to analysis. 0000034099 00000 n Gluconacetobacter diazotrophicus is a bacterium with a rod-like shape, has circular ends, and can be classified as a Gram-negative bacterium. 0000127870 00000 n 0000128095 00000 n CAB Direct is the most thorough and extensive source of reference in the applied life sciences, Extensive online help - available wherever you are in CAB Direct. 0000044289 00000 n 0000005744 00000 n 0000006297 00000 n 0000005823 00000 n 0000047581 00000 n startxref 0000003949 00000 n 0000126892 00000 n 0000053433 00000 n trailer 0000015981 00000 n CAB Direct [3] Gluconacetobacter diazotrophicus is a part of the Acetobacteraceae family and started out with the name, Saccharibacter nitrocaptans, however, the bacterium is renamed as Acetobacter diazotrophicus because the bacterium is found to belong with bacteria that are able to tolerate acetic acid. 0000005667 00000 n [2] In addition to being a part of the Acetobacter family, Gluconacetobacter diazotrophicus belongs to the Proteobacteria phylum, the Alphaprotebacteria class, and the Gluconacetobacter genus while being a part of the Rhodosprillales order. 0000041289 00000 n 0000005590 00000 n 0000044911 00000 n 0000057716 00000 n 0000052542 00000 n Gluconacetobacter diazotrophicus is a gram-negative bacterium, initially isolated as endophyte from Brazilian sugarcane plants8 and subsequently from sugarcane plants in other countries 6, 34. [1][2] The bacterium is known for stimulating plant growth and being tolerant to acetic acid. 0000003330 00000 n The leaves contained a smaller population (<10 3 g -1). CAB Direct provides 0000053931 00000 n session so others can sign in. %%EOF 0000006060 00000 n 0000124206 00000 n Gluconacetobacter diazotrophicus is also known for nitrogen fixation. 0000006218 00000 n 0000006535 00000 n 0000004733 00000 n [8] This microbe fights off Xanthomonas albilineans which is a pathogen found in sugar cane. 0000006455 00000 n 0000004655 00000 n The genome that was found to be closely sequenced with Gluconacetobacter diazotrophicus was the Pal5 genome. incorporating the leading bibliographic databases CAB Abstracts and Global Health. [2] The Pal5 genome was found to have 583 proteins that can be used to depict the possible “metabolic pathways” in Gluconacetobacter diazotrophicus. 0000004890 00000 n Gluconacetobacter diazotrophicus is a Gram-negative, acid tolerant, obligate aerobe and the cells are rod shaped with rounded ends (0.7–0.9 µm by 1–2 µm) having lateral or peritrichous flagella 0000006376 00000 n 112 108 0000041067 00000 n With one to three lateral flagella, and known to be found on sugarcane, Gluconacetobacter diazotrophicus was discovered in Brazil by Vladimir A. Cavalcante and Johanna Dobereiner. Like most websites we use cookies. 0000126572 00000 n 0000016173 00000 n 0000004498 00000 n 0000127986 00000 n <]>> 0000005981 00000 n 0000034965 00000 n 0000038730 00000 n means you agree to our use of cookies. [2] This feature allows the bacteria to work on nitrogen in the air in order for the correct amount of nitrogen can be received by plants. [10] You can now claim your publications on CAB Direct with your ORCID iD! [1][2] In addition to sugarcane, G. diazotrophicus has been found in different plants like coffee tree and pineapple. 0000006803 00000 n 0000035707 00000 n 0000054140 00000 n 0000034646 00000 n 0000003041 00000 n 0000000016 00000 n 0000127294 00000 n 0000004339 00000 n [2] The two plasmids found in the genome are pGD01 and pGD02 which contain 38,818 and 16,610 base pairs respectively.

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